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Why Choose The FOXP3 Antibody For Research

Posted by , on Sep, 2017

The FOXP3 antibody is a 431 amino acid protein and part of the forkhead/winged-helix family, highly conserved across mammals. It is necessary for normal immune homeostasis and is constitutively and stably expressed at high levels in CD25+CD4 positive regulatory T-Cells. It is found in low levels of CD4 positive and CD25 negative cells but is absent from CD4 negative and CD8 positive T-Cells. It may also be considered a master regulatory gene and a better market for regulatory T-cells. More Information The polyclonal version has no known clone, and the immunogen is a synthetic peptide that is derived from the c-terminus of the protein. The monoclonal version has a clone of SP97, and the immunogen is a synthetic peptide that corresponds to the c-terminus of the protein. Both antibodies have a Rabbit IgG isotype and an undetermined epitope. They both have a molecular weight of 50kDa and have both been tested on humans. Both types will also use the IHC application. Immunohistochemistry Application Procedure You can use paraffin-embedded or Formalin-fixed tissues for either version, and deparaffinized slides are essential. For the polyclonal FOXP3 antibody, you’ll want to dilute the antibody using a ratio of 1:200 when using the concentrated format. When dealing with the monoclonal version and the concentrated format, you’ll want to dilute the antibody using a ratio of 1:100. To retrieve the antigen, no matter which style you have, you’ll boil the tissue section in a 1mM EDTA with a pH of 8.0 for 10 minutes, allowing it to cool for 20 minutes to reach room temperature. You’ll also need to incubate for 30 minutes, as well. The positive control is the tonsil with cellular localization occurring in the nucleus. The FOXP3 antibody comes in two forms and can help you learn more about T-cells. Visit Spring BioScience now to find out more. 1 person likes this post. Like...

A Biologically Engineered Way To Fight Cancer

Posted by , on Oct, 2016

In terms of medical research, it seems that there isn’t much outside our grasp. We’re constantly finding new ways to cure diseases and eliminate many of the illnesses that have plagued humanity for its existence. By using engineered antibodies, scientists have discovered ways to even treat some untreatable diseases such as cancer. With the help of anti PD-L1 antibodies, patients found that it helps significantly more with battling cancer. PD-L1 is a protein in the body that has been identified by doctors as being associated with cancer and is often found inside of tumors. An antibody can be genetically engineered to block the production of this protein and in many cases cause the patient to go into remission. It’s not a comprehensive cure just yet, but it’s certainly a start. Minimized Side Effects When dealing with diseases that aren’t curable such as cancer, doctors often focus their efforts on extending the lifespan of the patient and ensuring a positive quality of life. Chemotherapy, while considered by many to be the most effective treatment for cancer, can often disrupt the patient’s way of life and make them feel even more ill. One of the advantages of anti PD-L1 antibodies however, is that they have very mild side effects when compared to that of chemotherapy, and can allow a patient to enjoy themselves a bit more without feeling nauseous or lethargic. A Fighting Chance A lot of people consider cancer to be a death sentence, but the truth is that in this day and age it really isn’t. With so many different treatment options available, getting good cancer treatment today is easier than any time before. Today cancer only bears a chance of death instead of being a downright death sentence. A PD-L1 inhibitor treatment can give a lot of patients, who express PD-L1 in their tumors, a far greater chance of survival. However, that’s also one of its shortcomings as it has been found that in patients who do not express PD-L1 in their tumors it has a greatly diminished effect. This doesn’t stop many doctors from prescribing the treatment though, as increasing your odds by any marginal amount is better than not investing the effort. For more information on anti PD-L1, contact Spring Bioscience online at  today. 2 people like this post. Like...

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