The serine/threonine-protein kinase B-raf, otherwise known as BRAF is part of the Raf family. Mutations in BRAF are quite common in both malignant and benign tumors in humans. The V600E mutation can account for most of the alternations, and such a mutation induces conformational changes of the active segment, which leads to constitutive kinase activity of the antibody, as well as the phosphorylation of any downstream targets. This mutation has been detected in a variety of situations, including melanoma, pleomorphic xanthoastrocytomas, papillary thyroid carcinoma, borderline ovarian cancer, Langerhans cell histiocytosis, ganglioglioma, pilocytic astrocytoma, and colorectal carcinoma.
More Information
Both the rat and mouse versions should only be used for research. The mouse version has a clone called VE1, and the rat version has a clone called pBR1. The immunogen for the mouse antibody is the synthetic peptide that represents mutated BRAF amino acid sequences from 596 to 606. The immunogen for the rat version is the full-length recombinant BRAF protein in humans. The isotype is either the mouse IgG2a or the rat IgG2a, and each has an undetermined epitope. Both versions have a molecular weight of 95 kDa, as well.
Applications
The mouse BRAF antibody should use the Immunohistochemistry application, but the rat version can use either IHC or Western Blotting, which is highly recommended but not tested.
You can find a variety of sizes for both versions, including 0.1 ml, 1.0 ml, and 0.5 ml concentrated versions. You may also find a 7.0 ml pre-diluted formulation, though it will depend on your dilution ratio to determine if you can use it. The mouse BRAF antibody does not include a pre-diluted formulation.
The BRAF antibody, both mouse and rat versions, can be used in a variety of ways to research other problems that humans face. Visit Spring Bioscience now for more information.
