The FOXP3 antibody is a 431 amino acid protein and part of the forkhead/winged-helix family, highly conserved across mammals. It is necessary for normal immune homeostasis and is constitutively and stably expressed at high levels in CD25+CD4 positive regulatory T-Cells. It is found in low levels of CD4 positive and CD25 negative cells but is absent from CD4 negative and CD8 positive T-Cells. It may also be considered a master regulatory gene and a better market for regulatory T-cells.
The polyclonal version has no known clone, and the immunogen is a synthetic peptide that is derived from the c-terminus of the protein. The monoclonal version has a clone of SP97, and the immunogen is a synthetic peptide that corresponds to the c-terminus of the protein. Both antibodies have a Rabbit IgG isotype and an undetermined epitope. They both have a molecular weight of 50kDa and have both been tested on humans. Both types will also use the IHC application.
Immunohistochemistry Application Procedure
You can use paraffin-embedded or Formalin-fixed tissues for either version, and deparaffinized slides are essential. For the polyclonal FOXP3 antibody, you’ll want to dilute the antibody using a ratio of 1:200 when using the concentrated format. When dealing with the monoclonal version and the concentrated format, you’ll want to dilute the antibody using a ratio of 1:100.
To retrieve the antigen, no matter which style you have, you’ll boil the tissue section in a 1mM EDTA with a pH of 8.0 for 10 minutes, allowing it to cool for 20 minutes to reach room temperature. You’ll also need to incubate for 30 minutes, as well. The positive control is the tonsil with cellular localization occurring in the nucleus.
The FOXP3 antibody comes in two forms and can help you learn more about T-cells. Visit Spring BioScience now to find out more.